Coding

Part:BBa_C100007:Design

Designed by: Matt Gethers   Group: MIT 20.109 Spring07   (2009-06-25)


ORF of the EHT1 odorant enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added biobricks prefix and suffix. Removed EcoRI site through SDM.


Source

Amplified via PCR from S. cerevisiae. Requires SDM to remove one EcoRI site.

References